Auxin regulated gene expression has been extensively studied and is known to involve active transcriptional regulation as well as protein degradation. One outstanding question in the field is how these gene expression changes are captured at the level of protein abundance. In order to address this knowledge gap, we have utilized global proteomics profiling approaches across tissues and time in Arabidopsis following auxin treatments. Using young 5 day-old seedlings we treated intact plants with a naturally occurring auxin, indole-3-acetic acid, for 30 min - 3 hours and then harvested tissue for profiling such that we were able to capture changes in protein abundance in seedlings, roots and hypocotyls. These data provide a complimentary picture to auxin-mediated transcriptional regulation and an avenue for future studies to examine novel proteins and their roles in auxin signaling during vegetative development. We are currently functionally characterizing several candidate proteins from these datasets using reverse genetics approaches.