In collaboration with Dr. Justin Walley (PLPM), we have recently characterized a large-scale catalog of ubiquitinated proteins from Arabidopsis roots using diGly affinity purification followed by mass spectrometry.
Auxin regulated gene expression has been extensively studied and is known to involve active transcriptional regulation as well as protein degradation. One outstanding question in the field is how these gene expression changes are captured at the level of protein abundance. In order to address this knowledge gap, we have utilized global proteomics profiling approaches across tissues and time in Arabidopsis following auxin treatments.
Auxin signaling is a key regulator of root morphogenesis in angiosperms, yet this pathway is understudied in maize and little is known regarding genetic factors that determine maize root architecture. Recent reports have demonstrated that root architecture can directly impact yield and drought resistance in maize. The overall objective of this project is to understand the key regulatory events governing auxin-mediated root development in maize using an integrated molecular genomics-to-phenotype approach.
Across the tree of life sugars are central energy signaling molecules. The post-embryonic developmental plasticity of plants relies on the ability of stem cell populations (termed meristems) to integrate environmental cues, such as carbon availability with hormone signaling for coordinated growth and developmental transitions. Auxin is one of the classical plant hormones in plant development and it is well appreciated that auxin influences many aspects of plant growth; how diverse growth responses are driven by one simple molecule is still an outstanding question in the field.