Publications

Li, Y., C. Wang, W. Cai, S. Sengupta, M. Zavortink, H. Deng, J. Girton, J. Johansen and K.M. Johansen (2017) H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation. Development 144 : 3232-3240.

Sengupta, S., U. Rath, C, Yao, M. Zavortink, C. Wang, J. Girton, K.M. Johansen and J. Johansen (2016) Digitor/dASCIZ has multiple roles in Drosophila development. PloS ONE 11 : e0166829.

Forer, A., K.M. Johansen and J. Johansen (2015) Movement of chromosomes with severed kinetochore microtubules.Protoplasma 252 : 775-781.

Wang, C.,Y. Li, W. Cai, X. Bao, J. Girton, J. Johansen and K.M. Johansen (2014) Histone H3S10 phosphorylation by the JIL-1 kinase in pericentric heterochromatin and on the 4th chromosome creates a composite H3S10phK9me2 epigenetic mark. Chromosoma 123 : 273-280.

Cai, W., C. Wang, Y. Li, C. Yao, L. Shen, S. Liu, X. Bao, P. Schnable, J. Girton, J. Johansen and K.M. Johansen (2014) Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 mediated histone H3S10 phosphorylation in Drosophila. Nucleic Acids Res.42 : 5456-5467.

Yao, C., C. Wang, Y. Li, Y. Ding, U. Rath, S. Sengupta, J. Girton, K.M. Johansen and J. Johansen (2014) The spindle matrix protein, Chromator, is a novel tubulin binding protein that can interact with both microtubules and free tubulin.  PLoS ONE 9 : e103855.

Li, Y., W. Cai, C. Wang, C. Yao, X. Bao, H. Deng, J. Girton, J. Johansen and K.M. Johansen (2013) Domain requirements of the JIL-1 tandem kinase for histone H3 serine10 phosphorylation and chromatin remodeling in vivo. J. Biol. Chem. 288 : 19441-19449.

Wang, C., C. Yao, Y. Li, W. Cai, X. Bao, J. Girton, J. Johansen and K.M. Johansen (2013) Evidence against a role for the JIL-1 kinase in H3S28 phosphorylation and 14-3-3 recruitment to active genes in Drosophila. PLoS ONE 8 : e62484.

Girton, J., C. Wang, J. Johansen and K.M. Johansen (2013) The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome. Fly 7: 129-133.

Yao, C., U. Rath, H. Maiato, D. Sharp, J. Girton, K.M. Johansen and J. Johansen (2012)  A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis.  Mol. Biol. Cell 23 : 3532-3541. [Supplementary Movies].

Wang, C., W. Cai, Y. Li, J. Girton, J. Johansen and K.M. Johansen (2012)  H3S10 phosphorylation by the JIL-1 kinase regulates H3K9 dimethylation and gene expression at the white locus in Drosophila.  Fly  6 : 93-97.

Yao, C., Y. Ding, W. Cai, C. Wang, J. Girton, K.M. Johansen and J. Johansen. (2012) The chromodomain-containing NH2-terminus of Chromator interacts with histone H1 and is required for correct targeting to chromatin. Chromosoma 121 : 209-220.

Wang, C., W. Cai, H. Deng, X. Bao, J. Girton, J. Johansen and K.M. Johansen (2011) The epigenetic H3S10ph mark is required for counteracting heterochromatic spreading and gene silencing in Drosophila.  J. Cell Sci. 124 : 4309-4317.

Wang, C., J. Girton, J. Johansen and K.M. Johansen (2011) A balance between euchromatic [JIL-1] and heterochromatic [SU(VAR)2-5 and SU(VAR)3-9] factors regulates position-effect variegation in Drosophila.  Genetics 188 : 745-748.

Johansen, K.M., A. Forer, C. Yao, J. Girton and J. Johansen (2011) Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix? Chromosome Res. 19 : 345-365.

Deng, H., W. Cai, C. Wang, S. Lerach, M. Delattre, J. Girton, J. Johansen and K.M. Johansen (2010) JIL-1 and Su(var)3-7 interact genetically and counterbalance each others' effect on position effect variegation in Drosophila. Genetics 185 : 1183-1192.

Boeke, J., C. Regnard, W. Cai, J. Johansen, K.M. Johansen, P.B. Becker, A. Imhof (2010)  Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1.  PloS ONE  : e10042.

Cai, W., Y. Jin, J. Girton, J. Johansen and K.M. Johansen (2010) Preparation of polytene chromo-some squashes for antibody labeling. J. Vis. Exp. https://www.jove.com/video/1748/preparation-drosophila-polytene-chromosome-squashes-for-antibody. [Quicktime Movie] [PDF]

Qi., H., C. Yao, W. Cai, J. Girton, K.M. Johansen and J. Johansen. (2009) Asator, a tau-tubulin kinase homolog in Drosophila localizes to the mitotic spindle.  Dev. Dynamics 238 : 3248-3256.

Ding, Y., C. Yao, M. Lince-Faria, U. Rath, W. Cai, H. Maiato, J. Girton, K.M. Johansen and J. Johansen. (2009) Chromator is required for proper microtubule spindle formation and mitosis in Drosophila.  Dev. Biol. 334 : 253-263.

Johansen, J. and K.M. Johansen (2009) The spindle matrix through the cell cycle in Drosophila.Fly 3 : 22-29.

Johansen, K.M., W. Cai, H. Deng, X. Bao, W. Zhang, J. Girton and J. Johansen (2009) Methods for studying transcription and epigenetic chromatin modification in Drosophila polytene chromosome squash preparations using antibodies.  Methods 48 : 387-397.

Lince-Faria, M., S. Maffini, B. Orr, Y. Ding, C. Florindo, C.E. Sunkel, A. Tavares, J. Johansen, K.M. Johansen and H. Maiato (2009)  Spatio-temporal control of mitosis by the conserved spindle matrix protein Megator. J. Cell Biol. 184 : 647-657.

Bao, X., W. Cai, H. Deng, W. Zhang, R. Krencik, J. Girton, J. Johansen and K.M. Johansen (2008) The COOH-terminal domain of the JIL-1 histone H3S10 kinase interacts with histone H3 and is required for correct targeting to chromatin.  J. Biol. Chem. 283 : 32741-32750.

Cai, W., X. Bao, H. Deng, Y. Jin, J. Girton, J. Johansen and K.M. Johansen (2008) RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila. Development 135 : 2917-2925.

Deng, H., X. Bao, W. Cai, M.J. Blacketer, A.S. Belmont, J. Girton, J. Johansen and K.M. Johansen (2008) Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila. Development 135 : 699-705.

Girton, J.R. and K.M. Johansen (2007) Chromatin structure and regulation of gene expression: the lessons of PEV in Drosophila. Adv. Genet. 61 :1-43.

Johansen, K.M and J. Johansen (2007) Cell and molecular biology of the spindle matrix. Int. Rev. Cytol. 263 : 155-206.

Deng, H., X. Bao, W. Zhang, J. Girton, J. Johansen and K.M. Johansen (2007) Reduced levels of Su(var)3-9 but not Su(var)2-5 (HP1) counteract the effects on chromatin structure and viability in loss-of-function mutants of the JIL-1 histone H3S10 kinase. Genetics 177 : 79-87.

Bao, X., H. Deng, J. Johansen, J. Girton and K.M. Johansen (2007) Loss-of-function alleles of the JIL-1 histone H3S10 kinase enhance position-effect-variegation at pericentric sites in Drosophila heterochromatin. Genetics 176 : 1355-1358.

Bao, X., J. Girton, J. Johansen and K.M. Johansen (2007) The lamin Dm0Ari3 allele acts as an enhancer of position effect variegation at the wm4 locus in Drosophila. Genetica 129 : 339-342.

Fabian, L., X. Xia, D.V. Venkitaramani, K.M. Johansen, J. Johansen D.J. Andrew, and A. Forer (2007) Titin in insect spermatocyte spindle fibres associates with microtubules, actin, myosin and the matrix proteins Skeletor, Megator, and Chromator. J. Cell Sci. 120 : 2190-2204.

Lerach, S., W. Zhang, X. Bao, H. Deng, J. Girton, J. Johansen and K.M. Johansen (2006) Loss-of-function alleles of the JIL-1 kinase are strong suppressors of position effect varigation of the wm4allele in Drosophila. Genetics 173 : 2403-2406.

Johansen, K.M. and J. Johansen (2006) Regulation of chromatin structure by histone H3S10 phosphorylation. Chromosome Res. 14 : 393-404.

Rath, U., Y. Ding, H. Deng, H. Qi, X. Bao, W. Zhang, J. Girton, J. Johansen, and K.M. Johansen (2006) The chromodomain protein, Chromator, interacts with JIL-1 kinase and regulates the structure of Drosophila polytene chromosomes. J. Cell Sci. 119 : 2332-2341.

Zhang, W., H. Deng, X. Bao, S. Lerach, J. Girton, J. Johansen and K.M. Johansen (2006) The JIL-1 histone H3S10 kinase regulates dimethyl histone H3K9 modifications and heterochromatic spreading in Drosophila. Development 133 : 229-235.

Lerach, S., W. Zhang, H. Deng, X. Bao, J. Girton, J. Johansen and K.M. Johansen (2005) The JIL-1 kinase, a member of the MSL complex, is necessary for proper dosage compensation of eye pigmentation in Drosophila. Genesis 43 : 213-215.

Bao, X., W. Zhang, R. Krencik, Y. Wang, J. Girton, J. Johansen, and K.M. Johansen (2005) The JIL-1 kinase interacts with lamin Dm0 and regulates nuclear lamina morphology of Drosophila nurse cells. J. Cell Sci. 118 : 5079-5087.

Deng, H., W. Zhang, X. Bao, J.N. Martin, J. Girton, J. Johansen and K.M. Johansen (2005) The JIL-1 kinase regulates the structure of Drosophila polytene chromosomes. Chromosoma 114 : 173-182.

Qi, H., U. Rath, Y. Ding, Y. Ji, M.J. Blacketer, J. Girton, J. Johansen and K.M. Johansen (2005) EAST interacts with Megator and localizes to the putative spindle matrix during mitosis in Drosophila. J. Cell Biochem. 95 : 1284-1291.

Qi, H., U. Rath, D. Wang, Y. Xu, W. Zhang, Y. Ding, M.J. Blacketer, M.R. Paddy, J. Girton, J. Johansen and K.M. Johansen (2004) Megator, an essential coiled-coil protein interacts with the putative spindle matrix during mitosis. Mol. Biol. Cell 15 : 4854-4865.

Rath, U., D. Wang, Y. Ding, Y-Z. Xu, M. Blacketer, J. Girton, J. Johansen and K.M. Johansen (2004) Chromator, an essential chromodomain protein interacts directly with the putative spindle matrix protein Skeletor. J. Cell. Biochem. 93 : 1033-1047.

Zhang, W., Y. Jin, Y. Ji, J. Girton, J. Johansen and K.M. Johansen (2003) Genetic and phenotypic analysis of alleles of the Drosophila chromosomal kinase JIL-1 reveals a functional requirement at multiple developmental stages. Genetics 165 : 1341-1354.

Johansen, K.M. and J. Johansen (2003) Studying nuclear organization in embryos using antibody tools. In Drosophila Cytogenetics Protocols. D.S. Henderson, Ed., Humana Press, Totowa, New Jersey. p. 215-234.

Zhang, W., Y. Wang, J. Long, J. Girton, J. Johansen and K.M. Johansen (2003) A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc-finger domain proteins interacts with the chromosomal kinase JIL-1. J. Biol. Chem. 278 : 11696-11704.

Johansen, K.M. and J. Johansen (2002) Recent glimpses of the elusive spindle matrix. Cell Cycle1 : 312-314.

Wang, Y., W. Zhang, Y. Jin, J. Johansen and K.M. Johansen (2001) The JIL-1 tandem kinase mediates histone H3 phosphorylation and is required for maintenance of chromatin structure in Drosophila. Cell 105 : 433-443.

Walker, D.L., D. Wang, Y. Jin, U. Rath, Y. Wang, J. Johansen and K.M. Johansen (2000) Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix. J. Cell Biol. 151 : 1401-1411.

Jin, Y., Y. Wang, J. Johansen and K.M. Johansen (2000) JIL-1, a chromosomal kinase implicated in regulation of chromatin structure, associates with the MSL dosage compensation complex. J. Cell Biol. 149 : 1005-1010.

Jin, Y., Y. Wang, D.L. Walker, H. Dong, C. Conley, J. Johansen and K.M. Johansen (1999) JIL-1: A novel chromosomal tandem kinase implicated in transcriptional regulation in Drosophila. Mol. Cell 4 : 129-135.

Johansen, K.M., J. Johansen, Y. Jin, D.L. Walker, D. Wang, and Y. Wang (1999) Chromatin structure and nuclear remodeling. Crit. Rev. Eukaryotic Gene Expr. 9 : 267-277.

Dong, H., Y. Jin, J. Johansen and K.M. Johansen (1999) Antibody identification, chromosome map assignment, and sequence analysis of a Rab escort protein homolog in Drosophila. Biochem. Biophys. Acta 1449 : 194-198.

Johansen, K.M. (1996) Dynamic remodeling of the nuclear architecture during the cell cycle. J. Cell. Biochem. 60: 289-296.

Johansen, K.M., J. Johansen, K.-H. Baek and Y. Jin (1996) Remodeling of nuclear architecture during the cell cycle in Drosophila embryos. J. Cell. Biochem. 63 : 268-279.