The bacterium, E. coli O157:H7, may cause illness or death as a result of its ingestion of undercooked meat products contaminated by it. Federal inspection agencies have mandated “zero-tolerance” for E. coli O157:H7 on beef carcasses. Although it is possible to detect single bacteria on a carcass, this cannot be done rapidly enough to monitor a line speed of several hundred carcasses an hour. We discovered, patented, and marketed a technology based on detecting the source of the bacterium: fecal material!