Plant Genome Research Outreach Program For Native Americans

         

Overview of Two months:

Three Students (Danielle Charley, Larry Morris Jr., Thurman Redhouse Jr.) are sent with the big question: is the sequence of Mo17 actually from the real Mo17. Some of these students had a little background in genetics and others had no background. Carolyn Lawrence and Candice Gardner along with the help of the Graduate students devised a plan to teach the students a little basic genetics before beginning the actual project. The project is broken up into 3 smaller projects to help the students understand the DNA sequencing.

Project 1: Genotyping; Sweet corn (1-2 weeks)

Purpose:
Students will learn some basic lab techniques. Basic lab techniques will include preparing stock solutions, PCR, preparing and running agarose DNA gels and etc.
Background:
Sweet corn may have similar alleles with B73 and Mo17.
Aim:
To show that IDP markers can find the similar alleles of sweet corn between B73 and Mo17.

Project 2.1: Genotyping on F2 and IBM RILs

Purpose:
Students can learn the genotyping method. IDP primers will serve as markers to find the genetic segregation of F2 ((B73 x Mo17)@), IBM RILs. Students will experience doing high-throughput DNA isolation and genotyping.
Background:
F2 seeds have genetic segregation ratio (1: 2: 1) and RILs population should have 1:1 segregation. Segregation will be analyzed on these two populations.
Aim:
To show that F2 seed have genetic segregation ration(1:2:1) and RIL seeds with ration 1:1..

Project 2.2: Genotyping on Rth2

Purpose:
To know more about genetic linkage and genetic segregation
Background:
rth2 mutants have significantly shorter root hair compared to wild type. Students will observe the phenotype of root hair of both mutants and wild types. Student will learn genetic linkage occurs in the mutant (rth2). This is an example to explain genetic linkage.
Experimental design:
To show rth2 mutant is located in chromosome 5. If we use IDP marker(s) that designed to use in chromosome 5, the result will show most bands form B73 and some double bands. If we use IDP marker that designed to use in chromosome 3, the result(s) will show genetic segregation ratio 1: 2: 1.

Project 3: Sequenom; Mo17(2-2.5 weeks)

Purpose:
Investigate whether Mo17 seeds from Hake's lab are the same as the seed available from the stock center at ISU. ~1,000K SNP markers will be used for Sequenom.
Aim:
To find out Hake's lab seeds are from the original source.


Home

Overview
2009 Participants
Research

Mo17 ??
-Problem
-Timeline
-genotyping(gel)
-genotyping(SNP)
-Results
Cell Culture
-Results
Participants

Danielle
Leslie
Thurman
Larry
Pictures

Research
PLAY
Elders
Sponsors

GWC
ISU
NSF
USDA