SNP Genotyping

Genotyping is a measurement of the variations in genes within members of a species. Missouri17(Mo17) as mentioned before was questioned if the sequenced Mo17 was the actual Mo17. Single nucleotied polymorphisms(SNP) is one of a common type of variation in genes. SNP is a single base pair mutation at a specific spot on a chromosone, most often on two alleles. SNP-typing Experiments were conducted following the Sequenom iPLEX Assay application note (Oeth, 2005) using ~ 1,000 SNPs. Genotyping data were acquired using the Sequenom Typer3.4 software. Only co-dominant markers that B73 and Mo17 have different SNP calls were used for the further analysis.