Plant Genome Research Outreach Program For Native Americans

Identifying the Mutant

As seen in the Outline, for most of the beginning part of the research done on Mo17 was in preparation for the actual SNP genotyping using the Sequenom machine. As a group the students learned how to do DNA extractions, run PCRs, make gels, and load samples into the gel to make gel pictures. PCR (polymerase chain reaction) is a technique used to amplify a certain piece of DNA and to create more copies of that single piece of DNA. The idea of this part of the project was to identify the mutant in the sweet corn DNA using the markers, on different chromosomes. Here are three gel pictures that were taken on the same day by each of the students. By using different markers, they were able to find out what which of the chromosomes held the mutant gene.

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