Nelli Lab
About
Nelli Lab specializes in developing advanced 3D cell culture models and diagnostics to address critical diseases affecting both animals and humans. We take pride in our innovative, high-throughput diagnostic solutions and in training the next generation of professionals. Our research focuses on understanding viral pathogenesis and host immune responses, particularly concerning coronaviruses and influenza viruses at the animal-human interface.
Our work is supported by over $3.5 million in federal, sponsored, and internal funding. We are actively looking for collaborative partners in academia and industry, as well as passionate students and post-doctoral researchers who are eager to contribute to the future of infectious disease control through collaborative research and impactful training opportunities.
Selected Publications
3D cell cultures
Three-dimensional (3D) cell cultures closely resemble the complex and physiological functions of animal organs. They have become valuable tools for studying organ development, modeling diseases, discovering drugs, personalizing medicine, and investigating host-specific immune responses toward pathogens.
Nelli Lab has established 3D cell cultures across various tissues and animal species to explore the critical molecular mechanisms at both transcriptome and proteomic levels. This research includes investigating species-specific immune responses to different pathogens, including zoonotic, with the goal of developing novel mitigation strategies.
Respiratory organoids Intestinal organoids
Mammary organoids Uterine organoids
Highthroughput qPCR testing services
Running qPCR assays for 12 genes across 128 triplicate samples on a 96-well plate typically requires approximately 16 plates, around 30 mL of master mix, and about 32 hours to complete two-hour qPCR reactions. This process may also lead to significant variability between plates. We offer SmartChip qPCR assays that can accomplish the same task in just 4 hours, using only about 2 mL of master mix and eliminating inter-plate variability, all at significantly lower prices.
Alternatively, we can conduct 10 plates of 96-well or 384-well qPCR assays overnight with our high-volume automation workflows.
Please contact us for more information.
Highthroughput qPCR testing and Laboratory Automation
Advancements in microfluidics, automation, robotic precision, vision systems, and decision-making algorithms have led to new solutions for high-throughput molecular diagnostics. Nelli Lab is exploring these cutting-edge laboratory diagnostic solutions to enhance future surge capacity testing needs.